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Gene Cloning Part 2: Making and Screening Gene Libraries: Objectives for Gene Cloning Part 2 Don Lee Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA Patricia Hain Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA Lesson Navigation Tips: - Click on 'Animations' button found to the left in order to view the animation which supplements this lesson. You can also click on the animation icon within the text. - Click once on figures to see enlarged versions. - Click once on words in color to bring up their definitions. At the conclusion of this lesson you should be able to... 1.) Describe the library screening process including why it is important in gene cloning. 2.) Determine which screening method would be appropriate for a given gene library. 3.) Compare traditional gene cloning with modern methods of automated DNA sequencing and database analysis techniques for gene cloning. Development of this lesson was supported in part by Cooperative State Research, Education, & Extension Service, U.S. Dept of Agriculture under Agreement Number 98-EATP-1-0403 administered by Cornell University and the American Distance Education Consortium (ADEC). Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.