Plant & Soil Sciences eLibraryPRO
Loading
control panel control panel
 

Transformation 1 - Plant Tissue Culture

Rate Me

Alternative to Tissue Culture

This chimeric alfalfa plant has a natural mutation that prevents chlorophyl production in some of the cells in the leaves.
Some genetic engineers have bypassed the tissue culture process by producing chimeric plants with the transgene in the germline cells. This means that they introduced the new gene into some but not all of the cells in a young plant.

Some of the cells in the seedling that geneticists have transformed are those that divide and develop into the pollen or egg producing tissues in the plant (the germline). Sexual progeny from these chimeric plants will then inherit the new gene and have one copy of the transgene in every one of their cells.

Another transformation method that could potentially bypass tissue culture is the transformation of pollen. In a species such as corn, the transformed pollen could be used in a cross with the hope that some of the progeny will inherit the transgene. Research on this method has produced limited results.

The most promising method for germline cell transformation is to transform germ cells that are within the developing flowers. This process works in the model plant arabidopsis simply by dipping the plant’s inflorescence in a solution of agrobacterium. The hope is to adopt this strategy to crop species.

Comments

Be the first to write a comment...

Control Panel cancel

Create activities for your moodle courses. Moodle Go to moodle
Select and group e-Library Lessons to create your own package... My Communities
Community Blogs Community Media

My Joined Communities

 
My Blogs - a journal of my thoughts... My Blogs
 
My Comments - my thoughts expressed as a feedback... My Comments
 
Classes that I am taking Registered Classes
Class Blogs Class Media
 
Check the scores of assesments that you have taken Taken Assessments
 
clear
Please confirm your selection.