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Transformation 1 - Plant Tissue Culture

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This lesson explains the technique of tissue culture as used in plant transformation. It discusses important issues, such as the use of selectable markers, genotype specificity, and tissue culture alternatives.

Overview and Objectives - Tissue Culture

Patricia Hain
Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA

Don Lee
Department of Agronomy and Horticulture at University of Nebraska-Lincoln, USA
 


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This lesson explains the technique of tissue culture as used in plant transformation. It discusses important issues, such as the use of selectable markers, genotype specificity, and tissue culture alternatives.


At the completion of this lesson, you should be able to:

  • List the six things necessary to successfully transform a plant.
  • Explain how the process of tissue culture is done.
  • Explain why tissue culture is a necessary part of producing a transgenic plant.
  • Describe genotype specificity and how it impacts genetic engineering.
  • List two alternatives to tissue culture sometimes used in genetic engineering.
  • Identify the role of selectable markers and how they are utilized in the transformation process.




Development of this lesson was supported in part by Cooperative State Research, Education, & Extension Service, U.S. Dept of Agriculture under Agreement Number 98-EATP-1-0403 administered by Cornell University and the American Distance Education Consortium (ADEC).
Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.

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